Journal: iScience

Article Title: Platelet DKK1 promotes tolerogenic dendritic cells and non-healing responses in cutaneous leishmaniasis

doi: 10.1016/j.isci.2026.115090

Figure Lengend Snippet: Decreased MPO + , CD11b + , and MHC class II + neutrophils in MyD88 (PKO) - and DKK1 (PKO) -infected mice (A–G) Six-week-old female WT-BALB/c, MyD88 (PKO) and DKK1 (PKO) mice were challenged with infective metacyclic promastigote (2 ×10 6 parasites, n = 5 per group) of L . major via the footpad. Non-infected BALB/c mice ( n = 10/2 feet per mouse) were given 0.9% NaCl saline. Cells were isolated from the footpads of all infected and non-infected mice at days 3 and 14 PI. Samples were analyzed by flow cytometry for MPO + , CD11b + , and MHC class II + neutrophils. A representative flow cytometry dot plot of MPO + , CD11b + , and MHC class II + neutrophils on day 3 is presented in . The percentage of MPO + , CD11b + , and MHC class II + cells in the different experimental groups is shown in column graphs (A, B, C, D, E, and F), while the absolute number of neutrophils obtained on day 3 PI is indicated (G). In all experiments, infected and non-infected BALB/c mice served as positive and negative controls, respectively. Results are presented as mean (±SEM) and are representative of two independent experiments. One-way ANOVA followed by Bonferroni’s post hoc test was used to analyze the data ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, non-significant ( p > 0.05).

Article Snippet: CD11b MicroBeads UltraPure, mouse , Miltenyi Biotec , Cat#130126725.

Techniques: Infection, Saline, Isolation, Flow Cytometry

Journal: iScience

Article Title: Platelet DKK1 promotes tolerogenic dendritic cells and non-healing responses in cutaneous leishmaniasis

doi: 10.1016/j.isci.2026.115090

Figure Lengend Snippet: Increased CD38 + macrophages and CD8α + dendritic cells in MyD88 (PKO) - and DKK1 (PKO) -infected mice (A–D) Six-week-old female WT-BALB/c, MyD88 (PKO) and DKK1 (PKO) mice were challenged with infective metacyclic promastigote (2 ×10 6 parasites, n = 5 per group) of L . major via the footpad. One week and two weeks post-infection, cells from the infected foot of each mouse in BALB/c, MyD88 (PKO) , DKK1 (PKO) , and non-infected BALB/c mice ( n = 10/2 feet per mouse) were harvested and counted for macrophages (A and B) and dendritic cells (C and D) by flow cytometry. (E–H) Two weeks post-infection, the infected foot from each mouse in BALB/c, MyD88 (PKO) , DKK1 (PKO) ( n = 5 per group), and non-infected BALB/c mice ( n = 10/2 feet per mouse) were examined for CD206 + and CD38 + macrophages (E and F), as well as CD11b + and CD8α + dendritic cells (G and H) by flow cytometry. Representative flow cytometry dot plots showing the analysis of CD206 + , CD38 + macrophages and CD11b + , CD8α + dendritic cells and a dot plot of each sample in all the experimental groups performed on day 14 PI are presented in . Results are presented as mean ± SEM and are representative of two independent experiments. Data analysis was done using one-way ANOVA followed by Bonferroni’s post hoc test ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and “ns” indicates non-significant.

Article Snippet: CD11b MicroBeads UltraPure, mouse , Miltenyi Biotec , Cat#130126725.

Techniques: Infection, Flow Cytometry

Journal: iScience

Article Title: Platelet DKK1 promotes tolerogenic dendritic cells and non-healing responses in cutaneous leishmaniasis

doi: 10.1016/j.isci.2026.115090

Figure Lengend Snippet: Decreased MPO + , CD11b + , and MHC class II + neutrophils in MyD88 (PKO) - and DKK1 (PKO) -infected mice (A–G) Six-week-old female WT-BALB/c, MyD88 (PKO) and DKK1 (PKO) mice were challenged with infective metacyclic promastigote (2 ×10 6 parasites, n = 5 per group) of L . major via the footpad. Non-infected BALB/c mice ( n = 10/2 feet per mouse) were given 0.9% NaCl saline. Cells were isolated from the footpads of all infected and non-infected mice at days 3 and 14 PI. Samples were analyzed by flow cytometry for MPO + , CD11b + , and MHC class II + neutrophils. A representative flow cytometry dot plot of MPO + , CD11b + , and MHC class II + neutrophils on day 3 is presented in . The percentage of MPO + , CD11b + , and MHC class II + cells in the different experimental groups is shown in column graphs (A, B, C, D, E, and F), while the absolute number of neutrophils obtained on day 3 PI is indicated (G). In all experiments, infected and non-infected BALB/c mice served as positive and negative controls, respectively. Results are presented as mean (±SEM) and are representative of two independent experiments. One-way ANOVA followed by Bonferroni’s post hoc test was used to analyze the data ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, non-significant ( p > 0.05).

Article Snippet: CD11b + monocytes were isolated and purified from female mouse bone marrow cells using CD11b MicroBeads UltraPure mouse magnetic bead (Miltenyi Biotec) according to the manufacturer’s protocol.

Techniques: Infection, Saline, Isolation, Flow Cytometry

Journal: iScience

Article Title: Platelet DKK1 promotes tolerogenic dendritic cells and non-healing responses in cutaneous leishmaniasis

doi: 10.1016/j.isci.2026.115090

Figure Lengend Snippet: Increased CD38 + macrophages and CD8α + dendritic cells in MyD88 (PKO) - and DKK1 (PKO) -infected mice (A–D) Six-week-old female WT-BALB/c, MyD88 (PKO) and DKK1 (PKO) mice were challenged with infective metacyclic promastigote (2 ×10 6 parasites, n = 5 per group) of L . major via the footpad. One week and two weeks post-infection, cells from the infected foot of each mouse in BALB/c, MyD88 (PKO) , DKK1 (PKO) , and non-infected BALB/c mice ( n = 10/2 feet per mouse) were harvested and counted for macrophages (A and B) and dendritic cells (C and D) by flow cytometry. (E–H) Two weeks post-infection, the infected foot from each mouse in BALB/c, MyD88 (PKO) , DKK1 (PKO) ( n = 5 per group), and non-infected BALB/c mice ( n = 10/2 feet per mouse) were examined for CD206 + and CD38 + macrophages (E and F), as well as CD11b + and CD8α + dendritic cells (G and H) by flow cytometry. Representative flow cytometry dot plots showing the analysis of CD206 + , CD38 + macrophages and CD11b + , CD8α + dendritic cells and a dot plot of each sample in all the experimental groups performed on day 14 PI are presented in . Results are presented as mean ± SEM and are representative of two independent experiments. Data analysis was done using one-way ANOVA followed by Bonferroni’s post hoc test ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and “ns” indicates non-significant.

Article Snippet: CD11b + monocytes were isolated and purified from female mouse bone marrow cells using CD11b MicroBeads UltraPure mouse magnetic bead (Miltenyi Biotec) according to the manufacturer’s protocol.

Techniques: Infection, Flow Cytometry